Related Experiment Video
Updated: Jul 11, 2026
Murine Model of Epicutaneously-Induced Immunomodulation
Published on: June 24, 2025
[Effects of Wenyang Chubi Decoction on connective tissue growth factor and collagen-I in a mouse model of
Xiao-ning Yan1, Jie Feng, Wen-bin Li
1Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an, Shannxi Province 710004, China.
Objective:
To study the effects of Wenyang Chubi Decoction (WYCBD), a compound traditional Chinese herbal medicine, on connective tissue growth factor (CTGF) and collagen-I (COL-I) in a mouse model of scleroderma.
Methods:
Scleroderma was induced in BALB/c mice by daily local injection of bleomycin for three weeks and the mice were randomly divided into untreated, WYCBD-treated and normal saline (NS) treated groups, with another group of BALB/c mice as normal control. WYCBD and NS were given orally for one month respectively. Histopathology in the skin and lungs of the mice were examined. The CTGF and COL-I expressions in the skin or skin lesions were detected by immunohistochemical Elivision assay.
Results:
The expression levels of CTGF and COL-I in the untreated group were significantly higher than those in the normal control group (P<0.05). Compared with the NS-treated group, the WYCBD-treated group had significant improvement in the skin and lung histopathology and remarkably decreased expression levels of CTGF and COL-I (P<0.05).
Conclusion:
Scleroderma mice showed high expressions of CTGF and COL-I in the skin. WYCBD had the effects of decreasing the CTGF and COL-I expressions and improving the skin fibrosis.
