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Real-time PCR genotyping of aldehyde dehydrogenase-2 using displacing probes

Yingying Gu1, Qingge Li

  • 1Molecular Diagnostics Laboratory, Department of Biomedical Sciences, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China.

Clinical Biochemistry
|September 15, 2007
PubMed
Abstract

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...