Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ca(2+)/Calmodulin-binding proteins from the C. elegans proteome.

Xinchun Shen1, C Alexander Valencia, Weiyi Gao

  • 1School of Pharmacy and Carolina Center for Genome Sciences, The University of North Carolina, Chapel Hill, NC 27599, United States.

Cell Calcium
|September 15, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Telerobotic partial nephrectomy and radical prostatectomy using a hybrid network: A single-center prospective experience.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2026
Same author

Clinical Outcomes of Orchiopexy and the Risk of Malignancy in Postpubertal Cryptorchid Patients.

Andrology·2026
Same author

Size-dependent ruthenium/ceria nanozymes synchronize catalytic ROS scavenging and electrostatic mtDNA sequestration for periodontitis therapy.

Journal of nanobiotechnology·2026
Same author

Bi-A@SR Nanoparticles Enable Tumor-Localized NO Release to Suppress HSP70 and Reverse Chemoresistance.

Advanced healthcare materials·2026
Same author

Resistant starch selectively depletes a putative pathobiont-enriched gut microbial module: evidence from multiple dietary fiber intervention cohorts.

Frontiers in nutrition·2026
Same author

Prevalence of pre-existing neutralizing antibodies to AAV5 and AAV8 in patients with Wilson's disease.

Molecular therapy. Advances·2026
Same journal

Calcium handling properties and arrhythmia vulnerability of cardiomyocytes from dystrophin-deficient mdx mice.

Cell calcium·2026
Same journal

Molecular actors of the Calcium signaling in the endothelial-to-mesenchymal transition: poorly explored therapeutic targets.

Cell calcium·2026
Same journal

High-throughput quantitation of pathogen-induced calcium signals captured through live-cell fluorescence microscopy.

Cell calcium·2026
Same journal

A model for the complete sequence of the human voltage-gated calcium channel (Ca<sub>V</sub>1.2) in a lipid bilayer.

Cell calcium·2026
Same journal

STIM1 but not STIM1L couples Ca<sup>2+</sup> extrusion to SOCE, promoting NFATc1 activation and human myotube growth.

Cell calcium·2026
Same journal

Orai1 mediates I<sub>CRAC</sub> and SOCE in human placental extravillous trophoblasts and contributes to cell viability, migration, and invasion.

Cell calcium·2026
See all related articles

Researchers identified new calcium-dependent calmodulin-binding proteins in C. elegans using mRNA-display. This method efficiently reveals novel protein interactions crucial for cellular signaling pathways.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Calmodulin (CaM) acts as a primary calcium (Ca2+) sensor regulating eukaryotic cellular processes.
  • Existing genome-wide protein-protein interaction maps in C. elegans have limited identified Ca2+/CaM-binding proteins.

Purpose of the Study:

  • To identify novel Ca2+-dependent calmodulin-binding proteins from the C. elegans proteome.
  • To characterize the binding properties and motifs of these newly found proteins.
  • To establish an efficient method for discovering Ca2+-binding protein partners.

Main Methods:

  • Utilized the mRNA-display technique, a genotype-phenotype conjugation method.
  • Screened a proteome library derived from adult C. elegans.
  • Characterized the Ca2+/CaM-binding properties and identified binding motifs.

Related Experiment Videos

Main Results:

  • Identified 9 known and 47 previously uncharacterized Ca2+-dependent CaM-binding proteins.
  • Detailed characterization of the binding properties and motifs of these proteins.
  • Demonstrated the efficacy of mRNA-display for identifying Ca2+-dependent protein interactions.

Conclusions:

  • The study significantly expands the repertoire of known Ca2+-dependent CaM-binding proteins in C. elegans.
  • The identified proteins and their motifs offer insights into Ca2+/CaM-mediated signaling pathways.
  • The mRNA-display method is a versatile tool for discovering Ca2+-binding partners in various signaling contexts.