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Updated: Jul 11, 2026

Live Imaging Characterization of Centromere Movements During Male Meiotic Prophase in Arabidopsis thaliana
Published on: October 24, 2025
Contribution of anaphase B to chromosome separation in higher plant cells estimated by image processing
Tomomi Hayashi1, Toshio Sano, Natsumaro Kutsuna
1Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba, 277-8562 Japan.
Abstract:
Anaphase can be categorized into the two subphases of anaphase A and B, but anaphase B has not been clearly described in higher plant cells. In this study, we time-sequentially followed the dynamics of chromosome segregation and spindle elongation in tobacco BY-2 cells using histone-red fluorescent protein (RFP) and green fluorescent protein (GFP)-tubulin, respectively. Construction of kymographs and determination of the positions of chromosomes and spindle edges by image processing revealed that anaphase B contributed to about 40% of the chromosome separation in distance, which is comparable with that in animal cells. These results suggest that higher plant cells potentially possess the process of anaphase B.
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