Related Experiment Video

Updated: Jul 11, 2026

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

PNAS-2: a novel gene probably participating in leukemogenesis

Hai-Rong Wang1, Chun-Hong Gu, Jian-Yi Zhu

  • 1Department of Hematology, Ren-Ji Hospital and Shanghai Institute of Hematology, Shanghai Jiao-Tong University School of Medicine, Shanghai, China.

Oncology
|September 15, 2007
PubMed
Abstract

Insights

The PNAS-2 gene acts as an anti-apoptotic factor, inhibiting programmed cell death. Its elevated expression is linked to acute leukemia development and progression, suggesting a role in leukemogenesis.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Hematology

Background:

  • Arsenic(4)sulfur(4) (As(4)S(4)) effectively treats acute promyelocytic leukemia, yet its mechanism is unclear.
  • PNAS-2, a human apoptosis-related gene, was previously identified through gene expression profiling.
  • The precise function of PNAS-2 in apoptosis and leukemia pathogenesis requires elucidation.

Purpose of the Study:

  • To investigate the role of PNAS-2 in apoptosis.
  • To determine the involvement of PNAS-2 in leukemogenesis.

Main Methods:

  • Utilized NB4 and U937 leukemia cell lines and clinical samples.
  • Employed RNA interference (RNAi) and RNA overexpression techniques to study PNAS-2 function.
  • Analyzed PNAS-2 expression in various tissues and patient samples using Northern blot and real-time PCR.

Main Results:

  • Functional studies revealed PNAS-2 acts as an anti-apoptotic gene.
  • PNAS-2 expression was significantly upregulated in de novo and relapsed acute leukemia.
  • PNAS-2 levels decreased to normal ranges in patients achieving complete remission, and were not elevated in carcinomas, indicating leukemia specificity.

Conclusions:

  • PNAS-2 functions as an anti-apoptotic gene.
  • PNAS-2 likely plays a role in the development of leukemia.

More Related Videos

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

Related Concept Videos

Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Pleiotropy01:33

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...