Related Experiment Videos
Purification and characterization of the N-methylcarbamate hydrolase from Pseudomonas strain CRL-OK
1Pesticide Degradation Laboratory, Agricultural Research Service, Beltsville, Maryland 20705.
Applied and Environmental Microbiology
|December 1, 1991
Abstract:
A unique cytosolic enzyme that hydrolyzes the carbamate linkage of the insecticide carbaryl (1-naphthyl N-methylcarbamate) was purified from extracts of Pseudomonas sp. strain CRL-OK. Substrates of the hydrolase include the N-methylcarbamate pesticides carbofuran and aldicarb but not the phenylcarbamate isopropyl m-chlorocarbanilate, the thiocarbamate S-ethyl N,N-dipropylthiocarbamate, or the dimethylcarbamate o-nitrophenyldimethylcarbamate.