Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparison of a quantitative microtiter method, a quantitative automated method, and the plate-count method for

M D Lee1, R E Wooley, J Brown

  • 1Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens 30602.

Avian Diseases
|October 11, 1991
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vitro action of combinations of antimicrobial agents with EDTA-tromethamine on Proteus vulgaris of a canine origin.

American journal of veterinary research·2013
Same author

An intestinal threadworm as a reservoir and intermediate host for Swine influenza virus : a confirmation and amplification of shope's syndrome.

The Journal of experimental medicine·2009
Same author

The evolving story of Mycobacterium tuberculosis clade members detected in fish.

Journal of fish diseases·2006
Same author

Effects of an extract of Serenoa repens on dogs with hyperplasia of the prostate gland.

American journal of veterinary research·2000
Same author

Inhibition of Salmonella typhimurium in the chicken intestinal tract by a transformed avirulent avian Escherichia coli.

Avian diseases·1999
Same author

Occurrence of Loma cf. salmonae in brook, brown and rainbow trout from Buford trout hatchery, Georgia, USA.

Diseases of aquatic organisms·1999

A new microtiter method effectively quantifies microbial complement resistance. This method, alongside automated and plate-count techniques, helps classify bacteria sensitivity to complement, crucial for understanding host-pathogen interactions.

Area of Science:

  • Microbiology
  • Immunology
  • Veterinary Science

Background:

  • The complement system is a critical component of innate immunity, mediating pathogen clearance.
  • Understanding microbial resistance or sensitivity to complement is vital for assessing virulence and developing treatments.
  • Accurate quantification of complement activity against microorganisms is essential for microbiological and immunological studies.

Purpose of the Study:

  • To describe and evaluate a novel quantitative microtiter method for assessing microbial complement resistance.
  • To compare the performance of the microtiter method against an automated system and the standard plate-count technique.
  • To classify avian Escherichia coli isolates based on their sensitivity or resistance to complement.

Main Methods:

Related Experiment Videos

  • A quantitative microtiter assay was developed to determine complement resistance.
  • Thirty avian Escherichia coli isolates were incubated with either native or heat-inactivated chicken plasma at 35°C.
  • Results were compared between the microtiter method, a quantitative automated system, and the standard plate-count technique.

    • Main Results:

    • The study successfully classified microorganisms into three groups: complement-sensitive, intermediate sensitivity, and complement-resistant.
    • Statistically significant correlations were observed among the three tested methods, despite absolute agreement not being achieved.
    • The microtiter method provides a viable alternative for quantifying complement resistance in microbial isolates.

    Conclusions:

    • The described microtiter method is a reliable tool for assessing microbial complement resistance and sensitivity.
    • This method aids in categorizing bacterial isolates based on their interaction with the complement system.
    • The findings contribute to a better understanding of host-pathogen dynamics, particularly in avian E. coli infections.