Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cultured astrocytes release proenkephalin.

D K Batter1, M H Vilijn, J Kessler

  • 1Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461.

Brain Research
|November 1, 1991
PubMed
Summary

Astrocytes release intact proenkephalin, unlike neurons which process it. This suggests glial proenkephalin has unique functions in the central nervous system.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Digital cognitive diagnostics (DiCoDi®) : A tablet-based cognitive test for people with and without hearing loss].

HNO·2026
Same author

Longitudinal reference ranges for Doppler blood velocity parameters of inferior adrenal artery in the human fetus.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2026
Same author

Reverse Løvset and Letellier rotational maneuvers: biomechanical parallels and important divergences (reply to letter to the editor).

American journal of obstetrics and gynecology·2025
Same author

Impact of delivery indication on levator ani muscle avulsion in forceps- and vacuum-assisted deliveries: prospective cohort study.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2025
Same author

A treadmill exercise reduced cardiac fibrosis, inflammation and vulnerability to ischemia-reperfusion in rat pristane-induced arthritis.

Life sciences·2024
Same author

The development of a hiPSC-based platform to identify tissue-dependencies of IDH1 R132H.

Cell death discovery·2023

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Neurons and astrocytes express proenkephalin mRNA, a precursor to opioid peptides.
  • Neuronal proenkephalin is processed into smaller peptides like Met-enkephalin and Leu-enkephalin.

Purpose of the Study:

  • To investigate the processing and release of proenkephalin in cultured astrocytes.
  • To compare proenkephalin processing in astrocytes versus neurons.

Main Methods:

  • Radioimmunoassay
  • Chromatographic analysis
  • Cultured neonatal rat brain astrocytes
  • Peptidase inhibitor experiments

Main Results:

  • Cultured astrocytes primarily contain unprocessed proenkephalin.
  • Astrocytes release intact proenkephalin into the culture medium.
  • Only small amounts of Met-enkephalin were detected in astrocytes.

Conclusions:

  • Astrocytes process and release proenkephalin differently than neurons.
  • Released proenkephalin may be cleaved extracellularly.
  • Intact proenkephalin from astrocytes suggests novel roles for opioid peptides in the central nervous system.

Related Experiment Videos