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Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
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Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
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The Two-State Receptor Model01:29

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Signal Transduction: Overview

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Typically, signal transduction involves three...

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Updated: Jul 11, 2026

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Published on: June 6, 2020

[Cytomics and receptor interaction].

Gero Brockhoff1

  • 1Institut für Pathologie, Universität Regensburg.

Verhandlungen Der Deutschen Gesellschaft Fur Pathologie
|September 18, 2007
PubMed
Summary

The erbB-receptor-tyrosine-kinase (RTK) system is complex, impacting cancer. Analyzing this system at the cytomic level offers new insights for personalized cancer therapy and drug targets.

Area of Science:

  • Oncology
  • Systems Biology
  • Cell Biology

Background:

  • The erbB-receptor-tyrosine-kinase (RTK) system, involving four receptors and numerous growth factors, is crucial for cell signaling.
  • Dysregulation of erbB-RTKs contributes to carcinogenesis, tumor progression, invasion, and metastasis.
  • Current diagnostics detect erbB-receptor gene amplification or protein overexpression but lack coexpression profiling.

Purpose of the Study:

  • To explore the potential of cytomics and systems biology in understanding the complex erbB-RTK system.
  • To investigate how analyzing the integrated erbB-receptor system on a single-cell level can improve disease monitoring and prognosis.
  • To identify novel therapeutic targets and enable precise patient stratification for individualized medicine.

Main Methods:

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  • Systems biology approach
  • Cytomics for analyzing cellular complexity
  • Single-cell level analysis of molecular function and dynamics

Main Results:

  • The complex interplay of erbB-receptors and growth factors dictates intracellular signaling diversity and specificity.
  • Understanding the erbB-RTK system at a cytomic level can lead to more precise disease follow-up and individual prognosis.
  • Comprehensive analysis is expected to reveal new drug targets and enable better patient stratification.

Conclusions:

  • Cytomics offers a novel approach to resolve the biocomplexity of the erbB-RTK system.
  • Analyzing the integrated erbB-receptor system on a single-cell level can enhance disease characterization and prediction.
  • This approach facilitates precise tumor patient stratification and the development of individualized, erbB-receptor-based therapeutic strategies.