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Published on: June 1, 2016
Characterization of vulnerable plaques by multiphoton microscopy
Magnus Borstad Lilledahl1, Olav Anton Haugen, Catharina de Lange Davies
1Norwegian University of Science and Technology, Department of Electronics and Telecommunications, O.S. Bragstads Plass 2A, 7491 Trondheim, Norway. magnus.b.lilledahl@iet.ntnu.no
Journal of Biomedical Optics
|September 18, 2007
Summary
Multiphoton microscopy (MPM) can measure fibrous cap thickness in vulnerable plaques, a key factor in cardiovascular disease deaths. This imaging technique aids in assessing plaque rupture risk by analyzing collagen and elastin content.
Area of Science:
- Biomedical Imaging
- Cardiovascular Research
- Materials Science
Background:
- Cardiovascular disease is the leading cause of death in the US.
- Vulnerable plaque rupture is a major cause of these deaths.
- Fibrous cap thickness (<65 microm) is critical for plaque stability.
Purpose of the Study:
- To evaluate multiphoton microscopy (MPM) for imaging vulnerable plaques.
- To accurately measure fibrous cap thickness.
- To assess plaque rupture propensity.
Main Methods:
- Multiphoton microscopy (MPM) was used to image plaque structure.
- Second harmonic generation (SHG) imaged collagen in the fibrous cap.
- Two-photon excited fluorescence (TPEF) imaged elastin in the core and artery wall.
- Collagen/elastin ratio was used for plaque detection.
Main Results:
- MPM achieved microm resolution imaging deeper than 65 microm.
- Distinct imaging of fibrous cap (collagen SHG) from core/artery (elastin TPEF).
- Plaque detection sensitivity of 65% and specificity of 81% using collagen/elastin ratio.
- Detailed collagen network structure analysis was possible.
Conclusions:
- MPM is effective for imaging and measuring fibrous cap thickness in vulnerable plaques.
- The technique provides insights into collagen structure, crucial for mechanical strength.
- MPM offers a potential method for assessing plaque rupture risk.
