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Updated: Jul 11, 2026

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
Published on: June 27, 2020
AF10-dependent transcription is enhanced by its interaction with FLRG
Stéphanie Forissier1, Diane Razanajaona, Anne-Sophie Ay
1Inserm, U590, Centre Léon Bérard, Université Lyon 1, Lyon, F-69003, France.
Follistatin-related gene (FLRG) interacts with AF10, enhancing its transcriptional activity. This reveals FLRG's nuclear role in regulating gene expression through AF10 homo-oligomerization.
Area of Science:
- Molecular Biology
- Gene Regulation
- Protein Interactions
Background:
- Follistatin-related gene (FLRG) is a glycoprotein similar to follistatin.
- FLRG binds TGF-beta superfamily members, regulating biological processes.
- Unlike follistatin, FLRG has nuclear localization, suggesting intracellular functions.
Purpose of the Study:
- To investigate the function of the nuclear form of FLRG.
- To identify proteins interacting with nuclear FLRG.
Main Methods:
- Yeast two-hybrid screen to identify FLRG-interacting proteins.
- Far-Western-blot and co-immunoprecipitation to confirm interactions.
- Transient transfection assays to assess transcriptional activity.
Main Results:
- Identified AF10 (ALL1 fused gene from chromosome 10) as an FLRG-interacting protein.
- Confirmed interaction between FLRG and AF10 using biochemical methods.
- Demonstrated that FLRG enhances AF10 homo-oligomerization and transactivation properties.
Conclusions:
- Nuclear FLRG functions as a novel transcriptional regulator.
- FLRG enhances AF10-mediated transcription by promoting AF10 homo-oligomerization.
- This mechanism likely facilitates co-activator recruitment for gene regulation.
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