Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microarray analysis of exstrophic human bladder smooth muscle.

Jason Hipp1, Karl-Erik Andersson, Tae Gyun Kwon

  • 1Department of Urology, Wake Forest Institute for Regenerative Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.

BJU International
|September 18, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling.

Drug discovery today·2016
Same author

A tunable hydrogel system for long-term release of cell-secreted cytokines and bioprinted in situ wound cell delivery.

Journal of biomedical materials research. Part B, Applied biomaterials·2016
Same author

Re: A Tumor Mitochondria Vaccine Protects against Experimental Renal Cell Carcinoma.

The Journal of urology·2016
Same author

Re: Endogenous Formaldehyde is a Hematopoietic Stem Cell Genotoxin and Metabolic Carcinogen.

The Journal of urology·2016
Same author

Re: Sphaeropsidin A Shows Promising Activity against Drug-Resistant Cancer Cells by Targeting Regulatory Volume Increase.

The Journal of urology·2016
Same author

Re: Sex Hormone-Dependent tRNA Halves Enhance Cell Proliferation in Breast and Prostate Cancers.

The Journal of urology·2016
Same journal

Cancer detection in the European Randomised Study of Screening for Prostate Cancer (ERSPC).

BJU international·2026
Same journal

Outcomes of maintenance BCG versus gemcitabine/docetaxel following BCG induction in NMIBC.

BJU international·2026
Same journal

A call for regimen agnostic circulating tumour DNA-guided adjuvant selection in muscle-invasive bladder cancer.

BJU international·2026
Same journal

Ablative radiotherapy in castration-resistant prostate cancer.

BJU international·2026
Same journal

Longitudinal circulating tumour DNA identifies patients at high risk of upstaging and recurrence in non-muscle-invasive bladder cancer.

BJU international·2026
Same journal

Venous thromboembolism after penile cancer surgery: a UK PeCaN study.

BJU international·2026
See all related articles

Bladder exstrophy smooth muscle cells (ESMCs) show genetic differences compared to healthy cells, with overexpressed inflammatory and underexpressed developmental genes, indicating immaturity.

Area of Science:

  • Regenerative Medicine
  • Developmental Biology
  • Genomics

Background:

  • Classical bladder exstrophy is a rare congenital disorder affecting bladder development.
  • Exstrophic bladders exhibit developmental immaturity and abnormal postnatal remodeling.
  • Smooth muscle cells from exstrophic bladders (ESMCs) are hypothesized to have distinct genetic profiles.

Purpose of the Study:

  • To compare gene expression in healthy bladder smooth muscle cells (SMCs) and exstrophic SMCs (ESMCs).
  • To identify genes over- and under-expressed in ESMCs.
  • To evaluate the molecular quality and therapeutic potential of ESMC tissue.

Main Methods:

  • Bladder smooth muscle biopsies were obtained from healthy individuals and patients with bladder exstrophy.

Related Experiment Videos

  • Cells were cultured in vitro, and total RNA was isolated.
  • Microarray analysis using Affymetrix U133A GeneChip was performed to compare gene expression profiles.

    • Main Results:

    • A genetic signature of 961 over-expressed and 432 under-expressed genes in ESMCs was identified.
    • Analysis revealed over-expression of inflammatory genes in ESMCs.
    • Under-expression of developmental genes was observed in ESMCs.

    Conclusions:

    • ESMCs are developmentally immature compared to healthy bladder SMCs, consistent with histological data.
    • The identified ESMC genetic signature offers potential therapeutic targets for inducing differentiation.
    • The genetic signature can serve as biomarkers for predicting clinical variability post-surgical closure.