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Related Experiment Videos

Invasive and noninvasive methods for studying pulmonary function in mice.

Thomas Glaab1, Christian Taube, Armin Braun

  • 11Department of Pulmonary Medicine, III. Medical Clinic, Johannes Gutenberg-University, Mainz, Germany. thomasglaab@web.de

Respiratory Research
|September 18, 2007
PubMed
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This review details in vivo lung function techniques for assessing airway responsiveness in genetically modified mouse models of asthma. It covers both invasive and noninvasive methods for characterizing pulmonary resistance and dynamic compliance.

Area of Science:

  • Pulmonary Physiology
  • Experimental Immunology
  • Genetically Modified Animal Models

Background:

  • Genetically altered mouse models are crucial for studying experimental asthma.
  • Characterizing functional outcomes of genetic manipulations requires robust in vivo lung function techniques.
  • Existing methods need clear evaluation for their application in asthma research.

Purpose of the Study:

  • To review and compare invasive and noninvasive methods for measuring airway responsiveness in mice.
  • To provide practical considerations for applying these techniques in laboratory settings.
  • To aid researchers in selecting appropriate methods for phenotyping mouse models of asthma.

Main Methods:

  • Invasive methods: assessment of pulmonary resistance (RL) and dynamic compliance (Cdyn) in anesthetized mice, low-frequency forced oscillations (LFOT).

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  • Noninvasive methods: head-out body plethysmography and barometric whole-body plethysmography in conscious mice.
  • Evaluation of technical principles, validation, applications, strengths, and weaknesses of each method.

    • Main Results:

    • Detailed descriptions of various classical and recent in vivo lung function measurement techniques are provided.
    • Comparative analysis highlights the advantages and limitations of invasive versus noninvasive approaches.
    • Practical considerations for applying these methods in mouse models are outlined.

    Conclusions:

    • A comprehensive understanding of available in vivo techniques is essential for accurate phenotyping of mouse asthma models.
    • Selection of the appropriate method depends on specific research questions and experimental conditions.
    • This review serves as a guide for researchers utilizing lung function measurements in murine asthma studies.