Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three-Dimensional DNA Nanostructure-Assisted Multiplexed Aptamer Assay for Simultaneous Detection of Endocrine-Disrupting Chemical Clusters.

ACS sensors·2026
Same author

Avoidance Behavior in Chinhai Spiny Newt Larvae: Responses to Visual and Chemical Cues from a Novel Predator.

Animals : an open access journal from MDPI·2026
Same author

Terrestrial hermit crab with electronic payloads for opportunistic roaming environmental sensing.

RSC advances·2025
Same author

Discrepancies in qPCR-based gene quantification and their dependencies on soil properties, inhibitor presence, and DNA extraction kit types.

RSC advances·2025
Same author

Development of an RPA-CRISPR/Cas12a-Based Rapid Diagnosis Strip for the Tangerine Pathotype of <i>Alternaria alternata</i>.

Microorganisms·2025
Same author

Probiotics beyond the farm: Benefits, costs, and considerations of using antibiotic alternatives in livestock.

Frontiers in antibiotics·2025

Related Experiment Video

Updated: Jul 11, 2026

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

Quantitative DNA hybridization in solution using magnetic/luminescent core-shell nanoparticles.

Ahjeong Son1, Dosi Dosev, Mikaela Nichkova

  • 1Department of Land, Air, and Water Resources, University of California Davis, One Shields Avenue, Davis, CA 95616, USA.

Analytical Biochemistry
|September 18, 2007
PubMed
Summary

This study presents a novel method for quantifying bacterial DNA using magnetic nanoparticles. The assay is rapid, simple, and avoids polymerase chain reaction, offering a new tool for environmental monitoring.

More Related Videos

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Related Experiment Videos

Last Updated: Jul 11, 2026

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Area of Science:

  • Nanotechnology
  • Biochemistry
  • Environmental Science

Background:

  • Methyl tertiary-butyl ether (MTBE) is a gasoline additive that contaminates groundwater.
  • Biodegradation of MTBE by bacteria is a crucial environmental remediation process.
  • Accurate quantification of specific bacterial DNA is essential for monitoring biodegradation efficiency.

Purpose of the Study:

  • To develop a rapid, non-polymerase chain reaction (PCR)-based method for quantifying bacteria that degrade MTBE.
  • To utilize nanoscale magnetic/luminescent core-shell particles for DNA quantification.
  • To demonstrate the specificity and potential environmental applications of the developed assay.

Main Methods:

  • Synthesis of Fe3O4/Eu:Gd2O3 core-shell nanoparticles via spray pyrolysis.
  • Biofunctionalization of nanoparticles with NeutrAvidin and immobilization of biotinylated probe DNA.
  • Hybridization of fluorescein isothiocyanate (FITC)-labeled target bacterial 16 S rDNA with immobilized probe DNA.
  • Magnetic separation of hybridized DNA complexes and fluorescence measurement using a spectrofluorometer.

Main Results:

  • Achieved linear quantification of target bacterial 16 S rDNA with a high correlation coefficient (R(2)=0.98).
  • Observed increased hybridization rates with increasing target DNA concentration.
  • Demonstrated assay specificity by differentiating between perfectly complementary and two-base mismatched target DNA.

Conclusions:

  • The developed nanoscale core-shell nanoparticle assay provides a sensitive and specific method for DNA quantification.
  • This approach offers a rapid, simple, and PCR-free alternative for quantifying bacteria involved in MTBE biodegradation.
  • The assay holds promise for environmental applications, including monitoring bioremediation processes.