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Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes
Published on: August 31, 2022
Rapid determination of viral RNA sequences in mosquitoes collected in the field
Yuki Kihara1, Tomomitsu Satho, Yuki Eshita
1Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
Journal of Virological Methods
|September 18, 2007
Abstract:
A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses.
