Related Experiment Video
Updated: Jul 11, 2026

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging
Published on: July 9, 2021
Histology safety: now and then
1Miramar, FL 33029-5926, USA. rjbuesca@yahoo.com
Abstract:
Histology safety usually focuses on general laboratory issues, but this article concentrates on the hazards affecting the individual histotech and their evolution in the last half a century. Using the information from a survey especially designed for the occasion, the hazards were divided into 4 groups, and their prevalence was expressed as percentages for national and foreign laboratories. All the laboratories received a "safety index" (SI) with an average value of 0.77 +/- 0.11 for 63 national laboratories and 0.69 +/- 0.13 for 22 foreign laboratories, these 2 averages being statistically different (P < .02). The historical evolution of the SI required answering the same questionnaire retrospectively, and so it was done for 17 laboratories with an SI average of 0.27 +/- 0.12 for 1955/1989 and 0.77 +/- 0.13, almost 3 times larger for 1990/2007, with improvement of all safety issues. The technological, organizational, and regulatory advances before 1989 showed an unremarkable effect on the SI, and the only circumstance considered as the driving force behind the almost triple increment of the SI during 1990/2007 was the awareness that the AIDS epidemic instilled in the minds and consciences of the medical laboratory personnel in general. Even after almost tripling the average SI value in 2007, national histology laboratories obtained a grade average of "C+" only, leaving room for improvement.
Insights
Histology laboratory safety for histotechs has significantly improved over 50 years, with the AIDS epidemic driving major safety index (SI) gains. Despite progress, histology labs still need enhanced safety measures.
Area of Science:
- Occupational Health
- Laboratory Science
- Histotechnology
Background:
- Histology laboratory safety often overlooks individual histotechnologist risks.
- A historical perspective on evolving hazards in histology is crucial for improving workplace safety.
Purpose of the Study:
- To assess the evolution of histology laboratory safety hazards over the past 50 years.
- To evaluate the impact of various factors on histology safety, using a Safety Index (SI).
Main Methods:
- A survey was conducted to identify and categorize histology laboratory hazards.
- A Safety Index (SI) was developed to quantify laboratory safety levels.
- Historical data was retrospectively collected to track SI changes from 1955 to 2007.
Main Results:
- The average SI for national histology laboratories was 0.77, and for foreign laboratories, it was 0.69.
- The SI significantly increased from 0.27 (1955/1989) to 0.77 (1990/2007), a nearly threefold improvement.
- The AIDS epidemic was identified as the primary driver for the substantial SI increase post-1989.
Conclusions:
- Histology laboratory safety has improved dramatically, particularly after 1990, largely due to increased awareness from the AIDS epidemic.
- Despite significant progress, national histology laboratories still exhibit safety levels equivalent to a 'C+', indicating a need for continued improvement.
Related Concept Videos
Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Standard Precaution
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
