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Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Published on: July 17, 2021
Laboratory production of 100 base pair DNA molecular weight markers
Microsugar Chang1, Jhih-Hao Wang, Han-Jung Lee
1Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
Journal of Biochemical and Biophysical Methods
|September 18, 2007
Abstract:
DNA molecular weight markers are routinely used in agarose gel electrophoresis. Here we report a method called PCR-synthesized marker (PSM) to generate DNA molecular rulers by PCR in the laboratory. This strategy can also be used to produce 100 bp RNA molecular weight markers by run-off transcription.

