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Related Experiment Videos

Oltipraz-induced decrease in the activity of cytosolic glutathione S-transferase in Schistosoma mansoni.

B Nare1, J M Smith, R K Prichard

  • 1Institute of Parasitology, McGill University, Ste-Anne de Bellevue, Québec, Canada.

International Journal for Parasitology
|December 1, 1991
PubMed
Summary

Oltipraz (OPZ) treatment significantly reduces glutathione S-transferase (GST) activity in Schistosoma mansoni, suggesting a direct interaction with GST isoenzymes may contribute to its antischistosomal effects.

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Area of Science:

  • Biochemistry
  • Parasitology
  • Pharmacology

Background:

  • Schistosoma mansoni is a parasitic flatworm causing schistosomiasis.
  • Glutathione S-transferases (GSTs) are crucial enzymes involved in detoxification and parasite survival.
  • Oltipraz (OPZ) is a compound investigated for its potential antischistosomal activity.

Purpose of the Study:

  • To investigate the effect of oltipraz (OPZ) on the activity and expression of glutathione S-transferase (GST) in Schistosoma mansoni.
  • To determine if OPZ affects specific GST isoenzymes and to elucidate the mechanism of OPZ's action.

Main Methods:

  • Enzyme activity assays for GST and glutathione peroxidase.
  • Protein quantification and SDS-polyacrylamide gel electrophoresis (SDS-PAGE).

Related Experiment Videos

  • Western blot analysis using isoenzyme-specific antisera.
  • Isoenzyme fractionation using chromatofocusing.

    • Main Results:

    • OPZ treatment progressively decreased GST activity in S. mansoni, but not peroxidase activities.
    • The reduction in GST activity exceeded the decrease in total GST protein.
    • Analysis revealed decreased levels of 26 and 28 kDa GSTs, with similar activity reductions across major isoenzymes (SmI, II, III).

    Conclusions:

    • OPZ and/or its metabolites directly interact with and inhibit GST activity in S. mansoni.
    • This inhibition affects multiple GST isoenzymes non-specifically.
    • The direct interaction and inhibition of GST by OPZ may be a key mechanism underlying its antischistosomal efficacy.