Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 11, 2026

An Automated Radiosynthesis of [68Ga]Ga-FAPI-46 for Routine Clinical Use
10:33

An Automated Radiosynthesis of [68Ga]Ga-FAPI-46 for Routine Clinical Use

Published on: May 24, 2024

Processing of generator-produced 68Ga for medical application.

Konstantin P Zhernosekov1, Dimitry V Filosofov, Richard P Baum

  • 1Institute of Nuclear Chemistry, Johannes Gutenberg-Universität Mainz, Mainz, Germany.

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
|September 18, 2007
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimizing FAPI homodimers for theranostic applications: The impact of the linker design in DOTAGA.Glu<sub>2</sub>.(FAPI)<sub>2</sub> and DOTAGA.PEG<sub>2</sub>.Glu.(FAPI)<sub>2</sub>.

European journal of nuclear medicine and molecular imaging·2026
Same author

[<sup>177</sup>Lu]Lu-edotreotide versus everolimus for gastroenteropancreatic neuroendocrine tumours (COMPETE): a phase 3, multicentre, randomised, open-label, superiority trial.

Lancet (London, England)·2026
Same author

Antitumor efficacy and safety of locoregional [<sup>177</sup>Lu]Lu-RM26 radionuclide therapy in glioblastoma.

European journal of nuclear medicine and molecular imaging·2026
Same author

Fibroblast Activation Protein-Targeted Radiopharmaceutical Therapy Using <sup>177</sup>Lu-, <sup>90</sup>Y-, and <sup>225</sup>Ac-Labeled 3BP-3940: First Experience in 21 Different Advanced Malignancies.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
Same author

Comparative Evaluation of [68Ga]Ga-DOTA.SA.FAPi and [18F]FDG PET/CT in Metastatic Breast and Lung Cancer: Semiquantitative, Volumetric and Prognostic Assessment.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

FAPI Tracer <i>en Vogue</i>: Evaluating [<sup>68</sup>Ga]Ga-DATA<sup>5m</sup>.SA.FAPi for Molecular Imaging of Pulmonary Fibrosis.

Pharmaceuticals (Basel, Switzerland)·2026

A new method efficiently purifies and concentrates Gallium-68 (68Ga) from radionuclide generators, enabling high-yield production of radiopharmaceuticals for PET imaging.

Area of Science:

  • Nuclear Chemistry
  • Radiopharmaceutical Chemistry
  • Medical Imaging

Background:

  • The (68)Ge/(68)Ga generator is a source of positron-emitting (68)Ga for radiopharmaceuticals.
  • Existing ionic (68)Ge/(68)Ga generators have limitations including large eluate volumes, high acidity, (68)Ge breakthrough, and impurities like Zn(II), Ti(IV), and Fe(III).

Purpose of the Study:

  • To develop an efficient method for processing (68)Ga eluates from radionuclide generators.
  • To optimize the purification and labeling of biomolecules with (68)Ga.

Main Methods:

  • Developed a post-elution processing system using a miniaturized cation-exchanger column with a hydrochloric acid/acetone eluent.
  • Preconcentrated and purified generator-derived (68)Ga eluates.
  • Labeled biomolecules, such as DOTATOC, with the purified (68)Ga.

More Related Videos

Automated Preparation of [68Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment
10:33

Automated Preparation of [68Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment

Published on: April 25, 2025

Investigations on the Ga(III) Complex of EOB-DTPA and Its 68Ga Radiolabeled Analogue
11:22

Investigations on the Ga(III) Complex of EOB-DTPA and Its 68Ga Radiolabeled Analogue

Published on: August 17, 2016

Related Experiment Videos

Last Updated: Jul 11, 2026

An Automated Radiosynthesis of [68Ga]Ga-FAPI-46 for Routine Clinical Use
10:33

An Automated Radiosynthesis of [68Ga]Ga-FAPI-46 for Routine Clinical Use

Published on: May 24, 2024

Automated Preparation of [68Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment
10:33

Automated Preparation of [68Ga]Ga-3BP-3940 on a Synthesis Module for PET Imaging of the Tumor Microenvironment

Published on: April 25, 2025

Investigations on the Ga(III) Complex of EOB-DTPA and Its 68Ga Radiolabeled Analogue
11:22

Investigations on the Ga(III) Complex of EOB-DTPA and Its 68Ga Radiolabeled Analogue

Published on: August 17, 2016

Main Results:

  • Achieved >97% recovery of (68)Ga activity in a small volume (0.4 mL) within 4 minutes.
  • Reduced (68)Ge breakthrough by a factor of 10(4) and impurities (Zn(II), Ti(IV), Fe(III)) by factors of 10(5), 10(2), and 10, respectively.
  • Obtained labeling yields of >95% for DOTATOC within 10 minutes, with overall yields of 70% and specific activities up to 450 MBq/nmol.

Conclusions:

  • The developed cation-exchange chromatography method is efficient for purifying and concentrating generator-derived (68)Ga.
  • The scheme ensures high yields and safe preparation of injectable (68)Ga-labeled radiopharmaceuticals for routine clinical PET applications.
  • This automated process facilitates the routine use of (68)Ga generators in clinical settings, potentially advancing clinical PET.