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Updated: Jul 11, 2026
09:16
Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
Published on: February 21, 2015
Array-based comparative genomic hybridization: clinical contexts for targeted and whole-genome designs
Swaroop Aradhya1, Athena M Cherry
1Department of Pathology, Stanford University School of Medicine, Palo Alto, California, USA. swaroop@genedx.com
Summary
Array-based comparative genomic hybridization (aCGH) offers higher resolution than traditional karyotyping for genome analysis. This review details targeted versus whole-genome array designs and their clinical applications in medical genetics.
Area of Science:
- Genomics
- Medical Genetics
- Molecular Biology
Background:
- Conventional G-banded karyotyping has limitations in genomic resolution.
- Array-based comparative genomic hybridization (aCGH) represents an advancement in genome analysis.
- Early genomic arrays utilized bacterial artificial chromosome (BAC) clones, evolving from targeted designs to whole-genome coverage.
Purpose of the Study:
- To review the evolution of array designs in aCGH.
- To differentiate between targeted and whole-genome array applications.
- To discuss the optimal clinical contexts for utilizing various array-based comparative genomic hybridization designs.
Main Methods:
- Review of array-based comparative genomic hybridization technologies.
- Comparison of targeted array designs (BAC clones) with whole-genome array designs (BAC clones and oligonucleotide probes).
- Analysis of array resolution capabilities, ranging from BAC-based to high-density oligonucleotide arrays (3-35 kb).
Main Results:
- Genomic arrays have progressed from targeted BAC clone arrays to high-density whole-genome oligonucleotide arrays.
- Different array designs offer varying resolutions suitable for specific clinical scenarios.
- Array-based comparative genomic hybridization is becoming a standard in diagnostic laboratories.
Conclusions:
- Array-based comparative genomic hybridization is transforming medical genetics diagnostics.
- Selecting the appropriate array design (targeted vs. whole-genome) is crucial for effective clinical application.
- This technology facilitates the discovery of novel genetic conditions linked to chromosomal anomalies.
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