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Updated: Jul 11, 2026

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

Genetic engineering of embryonic stem cells.

David M Suter¹, Michel Dubois-Dauphin, Karl-Heinz Krause

¹Department of Pathology and Immunology, Geneva Medical School, Switzerland.

Swiss Medical Weekly

|September 19, 2007

Summary

We developed new lentiviral vectors for embryonic stem cells. These vectors enable antibiotic selection and easy gene insertion, aiding transgenic cell line generation and neuronal engineering research.

Area of Science:

Stem cell biology
Molecular biology
Neuroscience

Background:

Embryonic stem cells (ESCs) are crucial for regenerative medicine and disease modeling.
Generating transgenic ESCs and studying neuronal differentiation requires efficient genetic tools.

Purpose of the Study:

To introduce a novel generation of lentiviral vectors designed for ESC applications.
To highlight the utility of these vectors for creating transgenic ESC lines and advancing neuronal engineering.

Main Methods:

Development of lentiviral vectors with antibiotic selection markers.
Facilitation of rapid insertion of promoters and genes of interest.
Application in monitoring neuronal emergence and differentiation from ESCs.

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Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

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Last Updated: Jul 11, 2026

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Published on: October 5, 2011

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

Main Results:

The novel lentiviral vectors are highly suitable for ESC manipulation.
Antibiotic selection allows for efficient generation of transgenic ESC lines.
Vectors enable real-time monitoring of neuronal differentiation from ESCs.

Conclusions:

This new lentiviral vector system offers a powerful tool for ESC research.
It significantly streamlines the creation of genetically modified ESCs.
The vectors are valuable for advancing studies in neuronal engineering and differentiation.