Related Experiment Video
Updated: Jul 11, 2026

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
Published on: August 23, 2014
Genetic engineering of embryonic stem cells
David M Suter1, Michel Dubois-Dauphin, Karl-Heinz Krause
1Department of Pathology and Immunology, Geneva Medical School, Switzerland.
Abstract:
We describe a novel generation oflentiviral vectors that are particularly well suited for work with embryonic stem cells. The possibility of selecting cell lines with antibiotics and the rapid insertion of any combination of promoters and genes of interest makes them a powerful tool in the generation of transgenic ES cell lines. This vector can also greatly facilitate studies aimed at the improvement of neuronal engineering from ES cells, by making it possible to monitor the emergence and differentiation of neurons.
Related Concept Videos
What is Genetic Engineering?
Embryonic Stem Cells
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells
In-vitro Mutagenesis
CRISPR
Induced Pluripotent Stem Cells

