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Updated: Jul 11, 2026

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
Published on: February 27, 2011
A stable isotope approach for the quantification of sewer infiltration
Oliver Kracht1, Markus Gresch, Willi Gujert
1Swiss Federal Institute of Aquatic Science and Technology, Eawag, 8600 Dübendorf, Switzerland. oliver.kracht@eawag.ch
Abstract:
Extraneous flows in wastewater collection systems are conventionally evaluated solely on the consideration of discharge hydrographs, which often involves a great degree of subjectivity and oversimplification. To obtain reliable information on the material fluxes within the urban environment, the use of intrinsic tracers can be the optimal choice. We demonstrate the successful use of naturally occurring stable isotopes of water (18O/16O and D/H) to accurately quantify extraneous discharge of groundwater in a combined sewer network. Fresh water supply from a distant hydrological regime provided usable isotopic separations between drinking water (proxy for real foul sewage) and local groundwater (proxy for sewer infiltration) of 1.8 per thousand in delta18O and 11.7 per thousand in 62H. Diurnal variation of wastewater isotopic composition reflected both the varying rates of foul sewage production and irregular dispersion effects in the pipe network. The latter suggest the existence of larger cumulative backwater zone volumes, which have not been attended to yet. Infiltrating groundwater contributed 39% (95% confidence interval = +/- 2.5%) of the total daily dry weather wastewater discharge. This paper discusses all relevant aspects for practical application of the method. It presents a comprehensive framework for uncertainty analysis and details on the detection and discrimination of possibly interfering effects.

