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RT-PCR-based cytochrome P450 expression profile of oral tissue samples
D Sarikaya1, I Chiba, C Bilgen
1Department of Bioengineering, Faculty of Engineering, University of Hokkaido, Japan.
Objective:
To analyse the expression of individual forms of cytochrome P450 (CYP) at the mRNA level in five homogenized oral buccal tissue samples from four individuals with or without oral malignancy.
Method:
Individual forms of CYPs were studied by reverse transcriptase-polymerase chain reaction (RT-PCR), using specific primers for CYPs 2B6, 2C, 2D6, 2E1, 3A3/4 and 3A5, and oral CYP expressions were compared with CYP expression in liver tissue.
Results:
Consistent expression of CYPs 2C, 2E1 and 3A5 was observed in oral buccal tissue at mRNA level.
Conclusions:
These particular CYPs have possible roles in the protection of the body against orally ingested xenobiotics as well as influence the bioavailability of therapeutic compounds.
