Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Toward a new era in sequencing.

Burkhard Ziebolz¹, Marcus Droege

¹Roche Diagnostics, Penzberg, Germany. burkhard.ziebolz@roche.com

Biotechnology Annual Review

|September 19, 2007

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

RESTORE: A Prospective Multinational Registry of Patients with Genetically Confirmed Spinal Muscular Atrophy - Rationale and Study Design.

Journal of neuromuscular diseases·2020

Same author

Economic burden of spinal muscular atrophy in the United States: a contemporary assessment.

Journal of medical economics·2019

Same author

Comparing the T scores from bone sonometer measurements in Hispanic and non-Hispanic white women.

Pharmacy practice·2014

Same author

The genome of melon (Cucumis melo L.).

Proceedings of the National Academy of Sciences of the United States of America·2012

Same author

Helicobacter pylori genome evolution during human infection.

Proceedings of the National Academy of Sciences of the United States of America·2011

Same author

A retrospective cohort study of economic outcomes and adherence to monotherapy with metformin, pioglitazone, or a sulfonylurea among patients with type 2 diabetes mellitus in the United States from 2003 to 2005.

Clinical therapeutics·2010

Same journal

Recent advances in the development of transgenic papaya technology.

Biotechnology annual review·2008

Same journal

The current status and future potential of personalized diagnostics: Streamlining a customized process.

Biotechnology annual review·2008

Same journal

Critical review and appraisal of published clinical literature: useful skill in biotechnology product development.

Biotechnology annual review·2008

Same journal

Free radical processes in green tea polyphenols (GTP) investigated by electron paramagnetic resonance (EPR) spectroscopy.

Biotechnology annual review·2008

Same journal

Ethnomedicines and ethnomedicinal phytophores against herpesviruses.

Biotechnology annual review·2008

Same journal

The application of low shear modeled microgravity to 3-D cell biology and tissue engineering.

Biotechnology annual review·2008

See all related articles

DNA sequencing, particularly the Sanger method, revolutionized medicine and biology by enabling genome analysis. However, this foundational technique is now nearing its technological limits.

Area of Science:

Genomics and Molecular Biology
Medical Research Applications

Background:

DNA sequencing is crucial for advancing medical and biological research.
The Sanger sequencing method, an electrophoresis-based technique, was fundamental to the Human Genome Project.
This method enabled the sequencing of both complete and fragmented genomes.

Purpose of the Study:

To highlight the significance of DNA sequencing in scientific discovery.
To acknowledge the historical importance and widespread use of the Sanger method.
To indicate the current limitations of the Sanger sequencing technology.

Main Methods:

Electrophoresis-based Sanger sequencing.
Genome and genome fragment analysis.

Main Results:

Related Experiment Videos

The Sanger method has been instrumental in achieving major scientific milestones, such as the Human Genome Project.
It facilitated the sequencing of extensive genetic material, including entire genomes.
The technique's widespread application has led to significant biological and medical insights.

Conclusions:

The Sanger sequencing method has reached its practical limitations.
Advancements in sequencing technology are necessary to overcome current challenges.
Continued innovation in sequencing is vital for future medical and biological breakthroughs.