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G and P rotavirus genotypes in stool samples from children in Teresina, State of Piauí

Carla Isabel Macedo1, Alice Christofoletti, Veridiana Munford

  • 1Institute Pasteur São Paulo, SP Brasil. cimacedo@usp.br

Revista Da Sociedade Brasileira De Medicina Tropical
|September 19, 2007
PubMed

Insights

Rotavirus strains in young children with diarrhea in Brazil showed unusual G and P genotype combinations. High percentages of mixed genotypes suggest potential gene reassortment and animal-to-human rotavirus transmission.

Area of Science:

  • Virology
  • Molecular Biology
  • Public Health

Background:

  • Rotavirus is a leading cause of severe diarrheal disease in infants and young children globally.
  • Understanding rotavirus genotype diversity is crucial for vaccine development and epidemiological surveillance.

Purpose of the Study:

  • To molecularly characterize G and P rotavirus genotypes in stool specimens from young children with diarrhea in Teresina, Piauí.
  • To investigate the prevalence of common and unusual rotavirus genotype combinations.

Main Methods:

  • Reverse transcriptase polymerase chain reaction (RT-PCR) was used for molecular characterization.
  • 123 stool specimens from children aged 0-2 years were analyzed.

Main Results:

  • The most common P genotypes were P[8] (17.1%) and P[1] (4.9%), with a high prevalence of mixtures (27.6%).
  • The most common G genotypes were G1 (25.2%) and G5 (13.8%), with mixtures found in 41.5% of samples.
  • Unusual combinations like P[1]G5 and P[1]+P[8]G5 were observed, along with frequent mixtures of human and animal rotavirus genotypes.

Conclusions:

  • The study identified a significant diversity of rotavirus G and P genotypes in the studied population.
  • The high frequency of mixed genotypes and unusual combinations suggests potential gene reassortment and interspecies transmission events.

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