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Updated: Jul 11, 2026

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
09:16

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Published on: December 14, 2015

Spontaneous transformation of a clonal population of dermis-derived multipotent cells in culture

Chunmeng Shi1, Yue Mai, Ying Zhu

  • 1Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, People's Republic of China. shicm1010@yahoo.com.cn

In Vitro Cellular & Developmental Biology. Animal
|September 19, 2007
PubMed

Insights

Adult multipotent stem cells can spontaneously transform in culture. This study identified the K-ras/mitogen-activated protein kinase kinase pathway

Area of Science:

  • Stem cell biology
  • Cancer research
  • Molecular mechanisms of transformation

Background:

  • Adult multipotent stem cells can spontaneously transform during long-term in vitro culture.
  • Understanding stem cell transformation is crucial for developing future therapeutic strategies.
  • The molecular basis of adult multipotent stem cell transformation remains poorly understood.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying the spontaneous transformation of adult multipotent stem cells.
  • To establish and characterize a tumorigenic cell line derived from rat dermis-derived multipotent cells (DMCs).

Main Methods:

  • Establishment of a tumorigenic cell line (TDMC1) from spontaneously transformed rat DMCs.
  • Subcutaneous inoculation of transformed cells into nude mice to assess tumor formation.
  • Analysis of molecular profiles using deoxyribonucleic acid (DNA) microarrays for both nontransformed and transformed cells.

Main Results:

  • A tumorigenic cell line, TDMC1, was successfully established from spontaneously transformed rat DMCs.
  • TDMC1 cells produced fibrous histocytoma-like tumors when inoculated into nude mice.
  • DNA microarray analysis revealed the overactivation of the K-ras/mitogen-activated protein kinase kinase signaling pathway in transformed cells.

Conclusions:

  • The K-ras/mitogen-activated protein kinase kinase signaling pathway plays a significant role in the malignant transformation of adult multipotent stem cells.
  • These findings provide insights into the molecular mechanisms driving stem cell transformation.
  • The established TDMC1 cell line serves as a valuable model for studying stem cell-related tumorigenesis.

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