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[Study on the application and evaluation of methods for gene and antigen detection in plague surveillance program]
Rong Hai1, Dong-zheng Yu, Xian-ming Shi
1State Kay Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Objective:
To apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.
Methods:
1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.
Results:
The overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.
Conclusion:
The new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.
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