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Updated: Jul 11, 2026
Modeling Chemotherapy Resistant Leukemia In Vitro
Published on: February 9, 2016
[Effect of daunorubicin and cytarabine on cell line NB4]
Zhong-Guang Luo1, Lu Zhong, Fang-Yuan Chen
1Department of Hematology, Renji Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200001, China.
Objective:
To explore whether daunorubicin (DNR) combined with cytosine arabinoside (Ara-C) and DNR alone have similar effect on acute promyelocytic leukemia (APL) cell line NB4 and acute myeloblastic leukemia cell line HL-60 in vitro.
Methods:
Cell morphology, cells viability, and cell apoptosis (Annexin-V by flow cytometry assay) were analysed.
Results:
After incubation with DNR plus Ara-C for 24 hours,NB4 cell viability [(36.75 +/- 3.82)%] (n = 6) and cell apoptosis rate [(21.24 +/- 5.82)%] (n = 3) did not change significantly compared to that treated with DNR alone for 24 hours [(35.73 + 6.28 )%, (22.55 +/- 3.26)%, respectively] (P > 0.05). However, HL-60 cell viability [(67.17 +/- 2.07)%] and cell apoptosis rate [(48.05 +/- 0.92)%] changed significantly in DNR plus Ara-C group compared with DNR alone [(63.31 +/- 1.80)% ,(41.51 +/- 0.89)%, respectively] (P < 0.01 and < 0.05, respectively).
Conclusion:
DNR plus Ara-C and DNR alone have similar effect on NB4 cells, but have different effect on HL-60 cells.
