Related Experiment Video

Updated: Jul 11, 2026

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
06:41

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

Analysis of the canine mdr1-1Delta mutation in the dog breed Elo

S Fecht1, A Wöhlke, H Hamann

  • 1Institute of Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Bünteweg 17p, 30559 Hannover, Germany.

Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine
|September 20, 2007
PubMed

Insights

The canine multidrug resistance gene (MDR1) deletion mutation, linked to drug sensitivity in breeds like Collies, was not found in the German Elo dog breed. This suggests the mutation is absent in the Elo population.

Area of Science:

  • Veterinary Genetics
  • Pharmacogenomics

Background:

  • A specific deletion mutation in the canine multidrug resistance gene (MDR1) is known to cause sensitivity to certain drugs.
  • This MDR1 mutation is prevalent in dog breeds of the Collie lineage, including Collies, Australian Shepherds, and Old English Sheepdogs.

Purpose of the Study:

  • To investigate the presence of the mdr1-1Delta mutation in the German dog breed Elo.
  • To determine if this drug sensitivity-associated mutation has been inherited by the Elo breed, which has Old English Sheepdogs in its ancestry.

Main Methods:

  • DNA was extracted from 177 blood samples representative of the Elo dog breed.
  • A polymerase chain reaction (PCR) based method followed by polyacrylamide gel electrophoresis was employed to detect the mdr1-1Delta mutation.

Main Results:

  • The mdr1-1Delta allele was not detected in any of the 177 Elo dogs tested.
  • The estimated probability of the mdr1-1Delta allele, originating from the Old English Sheepdog, segregating within the Elo population is extremely low (3.68 x 10^-17).

Conclusions:

  • The investigated Elo dog population does not carry the mdr1-1Delta mutation.
  • This finding indicates that the Elo breed is likely free from the MDR1-associated drug sensitivity mutation found in Collie-lineage dogs.

More Related Videos

Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy10:30

Multi-exon Skipping Using Cocktail Antisense Oligonucleotides in the Canine X-linked Muscular Dystrophy

Published on: May 24, 2016

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Related Concept Videos

Incomplete Dominance01:43

Incomplete Dominance

Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.
Epistasis Analysis01:09

Epistasis Analysis

Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...