Related Experiment Video
Updated: Jul 11, 2026

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
Published on: March 26, 2019
Duration and timing of sensitivity related to bleaching
William D Browning1, John S Blalock, Kevin B Frazier
1Department of Oral Rehabilitation, School of Dentistry, Medical College of Georgia, Augusta, GA, USA. wbrownin@mail.mcg.edu
Problem:
Reports of sensitivity vary greatly from one study to another, probably because studies are small. Generally, only the percentage of subjects is reported.
Purpose:
This study reports sensitivity using a large database. We investigated the source, duration, and timing of sensitivity during 14 days of active bleaching.
Materials And Methods:
One hundred and seventy-two people recorded sensitivity from any of the five sources on a daily basis.
Results:
No one withdrew from the study because of sensitivity. Forty-seven percent of participants experienced sensitivity. Seventy-seven percent had sensitivity of 3 or fewer days. Temperature sensitivity tended to occur later in the 14-day bleaching cycle, and hot and cold sensitivity tended to occur together.
Conclusions:
There was great variability in sensitivity levels from person to person. Temperature sensitivity tended to occur later in the active phase of bleaching, whereas irritation of the tongue tended to occur earlier.
Clinical Significance:
The results from a large group of people are more likely to include a wide cross-section of the population sampled. This data provides practitioners with a better estimate of what their patients are likely to experience. It is estimated that, during 2 weeks of active bleaching, 77% of people will experience 3 or fewer days of sensitivity. On average, sensitivity is short-lived, thus making it is easy to underestimate the importance of discussing sensitivity with patients considering bleaching. However, for some, the duration of sensitivity is much greater and has a very negative impact on satisfaction.
Related Concept Videos
Desensitization and Tachyphylaxis
Several...
Pigmentation
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
Photoreceptors and Visual Pathways

