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Related Experiment Videos

Myosin V stepping mechanism.

Giovanni Cappello1, Paolo Pierobon, Clémentine Symonds

  • 1Institut Curie, Centre National de la Recherche Scientifique Unité Mixte de Recherche 168, 75231 Paris, France. giovanni.cappello@curie.fr

Proceedings of the National Academy of Sciences of the United States of America
|September 20, 2007
PubMed
Summary
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Myosin V motors take 36-nm steps using a three-stage mechanism involving longitudinal and vertical movements. This process, observed with traveling wave tracking, reveals details of myosin V

Area of Science:

  • Biophysics
  • Molecular Biology
  • Cellular Motor Proteins

Background:

  • Myosin V is a motor protein crucial for intracellular transport.
  • Understanding its stepping mechanism is key to deciphering cellular cargo movement.

Purpose of the Study:

  • To elucidate the step mechanism of myosin V using advanced tracking.
  • To characterize the dynamics of myosin V during its 36-nm step.

Main Methods:

  • Traveling wave tracking combined with optical tweezers.
  • High-resolution (nanometer) and high-temporal (microsecond) observation of particle movement.
  • Alignment and averaging of numerous single-molecule events.

Main Results:

  • The 36-nm step occurs in three distinct stages: a 5-nm prestep, a 23-nm fast motion with vertical displacement, and diffusion to the next binding site.

Related Experiment Videos

  • Myosin V exhibits coupled longitudinal and vertical motion during stepping.
  • Step duration is influenced by ATP concentration.

    • Conclusions:

    • A three-stage model explains the myosin V stepping mechanism.
    • The observed dynamics provide insights into the processivity and function of myosin V.
    • This study advances our understanding of molecular motor mechanics.