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Connector inversion probe technology: a powerful one-primer multiplex DNA amplification system for numerous
Michael S Akhras1, Magnus Unemo, Sreedevi Thiyagarajan
1Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America.
We developed a novel one-primer multiplex amplification system called Connector Inversion Probe (CIPer) for pathogen genetic analysis. This versatile tool enables pathogen diagnosis, typing, and antibiotic resistance screening, enhancing molecular diagnostics.
Area of Science:
- Molecular Biology
- Genetics
- Pathogen Diagnostics
Background:
- Existing molecular assays have limitations in multiplexing capabilities.
- There is a need for versatile genetic tools for pathogen identification and resistance profiling.
Purpose of the Study:
- To develop a novel one-primer multiplex amplification system.
- To demonstrate the utility of the Connector Inversion Probe (CIPer) technology for pathogen diagnosis, typing, and antibiotic resistance screening.
Main Methods:
- Combined Molecular Inversion Probe (MIP) components with a gap-filling strategy.
- Developed a Connector Inversion Probe (CIPer) based assay.
- Applied CIPer for Human Papillomavirus (HPV) genotyping and Neisseria gonorrhoeae antibiotic resistance mutation screening.
Main Results:
- Successfully created a versatile one-primer multiplex amplification system.
- Demonstrated proof-of-concept for pathogen diagnosis and typing (HPV).
- Showcased utility for antibiotic resistance screening (N. gonorrhoeae).
Conclusions:
- Connector Inversion Probe (CIPer) technology offers a powerful and versatile platform for molecular diagnostics.
- CIPer has potential for integration with advanced techniques like digital amplification and next-generation sequencing.
- Future applications include two-dimension informational barcodes (MMPs) and user-friendly probe design software.
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