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Published on: September 25, 2019
Establishment and primary application of a mouse model with hepatitis B virus replication
Feng-Jun Liu1, Li Liu, Fang He
1Center of Infectious Diseases, Division of Molecular Biology of infectious Diseases, National Key Laboratory of Biotherapy (Sichuan University), West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China.
World Journal of Gastroenterology
|September 20, 2007
Summary
Researchers developed a rapid mouse model for hepatitis B virus (HBV) replication using hydrodynamic transfection. This model effectively demonstrated the inhibitory effects of polyinosinic-polytidylin acid (polyIC) on HBV replication.
Area of Science:
- Virology
- Hepatology
- Animal Models
Background:
- Hepatitis B virus (HBV) infection is a major global health concern.
- Developing efficient animal models is crucial for studying HBV replication and testing antiviral strategies.
Purpose of the Study:
- To establish a rapid and convenient animal model for HBV replication.
- To utilize this model to investigate the inhibitory effect of polyinosinic-polytidylin acid (polyIC) on HBV replication.
Main Methods:
- Hydrodynamic in vivo transfection of HBV-replication-competent plasmid into BALB/C mice.
- Analysis of HBV DNA replication intermediates, HBcAg, and HBsAg expression in the liver.
- Assessment of serum HBsAg and HBeAg levels.
- Comparison of HBV replication inhibition by polyIC versus phosphate-buffered saline (PBS) treatment.
Main Results:
- HBV DNA replication intermediates were detectable by day 1 and abundant by days 3-4 post-transfection.
- Hepatitis B core antigen (HBcAg) and hepatitis B surface antigen (HBsAg) expression mirrored DNA replication patterns.
- PolyIC treatment resulted in lower levels of HBV intermediate DNA compared to PBS controls.
Conclusions:
- A rapid and convenient mouse model for high-level HBV replication was successfully established.
- This model is effective for evaluating the antiviral potential of compounds like polyIC.
- The developed model serves as a valuable tool for future HBV genome functional studies.
