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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
[Study on separation and purification of total flavones from Smilax china by macroporous absorption resin]
Wen-xia Li1, Yan Yan, Xiao-chuan Ye
1Department of Chemistry, Wuhan 430074, China.
Objective:
To investigate the technological parameters of the purification process of total flavones from Smilax china with macroporous absorption resin.
Method:
The technical process for purification of total flavones with the optimum macroporous absorption resin was screened by yield of total flavones product.
Result:
The D140 macroporous absorption resin had the best separating efficiency when the flavones content in the liquid was 0.5 g x mL(-1) equivalent to raw material, the volume of drug 18 BV (resin bad volume) with the adsorption-power 2 BV x h(-1), and the volume of 60% (mL x mL(-1)) ethanol as eluant 5-10 BV (resin bad volume) with desorption-power 1 BV x h(-1). The obtained flavones product has total flavones recovery rate of 84.72%.
Conclusion:
The treatment of regenerated resin is easy, this method is advisable.
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