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Related Experiment Videos

Effect of temperature decrease on the microbial population and process performance of a mesophilic anaerobic

I Bohn1, L Björnsson, B Mattiasson

  • 1Department of Biotechnology, Lund University, P.O. Box 124, SE-22100 Lund, Sweden.

Environmental Technology
|September 21, 2007
PubMed
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Lowering anaerobic digestion temperatures from 33°C to 18°C improved methane production rates in crop residue digestion. Microbial community shifts, particularly an increase in Bacteria, facilitated adaptation and enhanced biogas yields.

Area of Science:

  • Environmental Science
  • Biotechnology
  • Microbiology

Background:

  • Anaerobic digestion (AD) is a key technology for converting crop residues into biogas.
  • Temperature significantly influences microbial community structure and function in AD processes.
  • Understanding microbial adaptation to mesophilic (33°C) and lower temperatures is crucial for optimizing AD efficiency.

Purpose of the Study:

  • To investigate the impact of decreasing temperature on the anaerobic digestion of crop residues.
  • To analyze microbial population dynamics during adaptation to lower temperatures.
  • To determine optimal temperature ranges for methane production from crop residues.

Main Methods:

  • Laboratory-scale continuously stirred fed-batch reactors were operated at 12°C, 18°C, and 33°C.

Related Experiment Videos

  • Fluorescence in situ hybridization (FISH) was used to monitor microbial community changes.
  • Methane production rates and hydrolysis, acidogenesis, and methanogenesis were assessed.

    • Main Results:

    • Stable methane production was achieved at 18°C after microbial adaptation, with yields of 151 ml CH4/g VS added.
    • Methane production rates at 25°C (310 ml CH4/l/day) and 37°C (366 ml CH4/l/day) exceeded those at 33°C (215 ml CH4/l/day).
    • Hydrolysis decreased below 25°C, with acidogenesis and methanogenesis becoming rate-limiting below 16°C.
    • Adaptation to 18°C involved an increase in Bacteria to total prokaryotes ratio and shifts in Archaea populations (increased Methanomicrobiales and Methanosarcinaceae, decreased Methanosaeta).

    Conclusions:

    • Lowering temperatures to 18°C can enhance methane production from crop residues through microbial adaptation.
    • Bacterial enrichment appears to be a key adaptation mechanism for improved AD performance at lower temperatures.
    • Temperature management is critical for optimizing anaerobic digestion efficiency and microbial community stability.