Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Patterned biofunctional poly(acrylic acid) brushes on silicon surfaces.

Rong Dong1, Sitaraman Krishnan, Barbara A Baird

  • 1Department of Chemistry, Materials Science and Engineering, and Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.

Biomacromolecules
|September 21, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Grafting polymer brushes from nylon surfaces <i>via</i> hydrogen atom transfer.

Chemical science·2026
Same author

Enhancement Strategies for Bioelectrocatalytic Conversion of Organic Waste.

Chem & bio engineering·2026
Same author

Surface-Initiated Hydrogen Atom Transfer Reversible Addition-Fragmentation Chain Transfer Polymerization from Isotactic Polypropylene.

Macromolecules·2026
Same author

ADAM8 Deficiency in Macrophages Alleviates Vascular Calcification in Chronic Kidney Disease.

Frontiers in bioscience (Landmark edition)·2026
Same author

Safeguarding a Flagship Species: Integrated Surveillance of Cross-Species Pathogen Transmission in Giant Panda Ecosystems.

Ecology and evolution·2026
Same author

Emergence of magnetic monopole-like behavior in iron oxide nanoparticles grafted with chiral brushes: a chiral induced spin selectivity manifestation.

Materials horizons·2026
Same journal

Ligno-Suberized Fruit Periderm as a Mechanically Reinforced Biomacromolecular Composite.

Biomacromolecules·2026
Same journal

Effect of Hydrophilic Brush Length and Hydrophobic Chain on Biodistribution of Polymethacrylate-Based Statistical Copolymers.

Biomacromolecules·2026
Same journal

Multicomponent Micelles with Boosted Stability of Iminoboronates.

Biomacromolecules·2026
Same journal

Stiffening and Toughening Protein Hydrogels by Tuning Electrostatic Interactions.

Biomacromolecules·2026
Same journal

<i>In Situ</i> Bulk and Interfacial Interlocking-Induced Highly Dynamically Entangled Hydrogel of Myocardium-Matching Mechanics, Electrophysiological Functions, and Robust Tissue Adhesion for Cardiac Repair.

Biomacromolecules·2026
Same journal

Eutectogel Electrodes with Self-powered Capability for Flexible Electrophysiological Sensor.

Biomacromolecules·2026
See all related articles

This study presents a mild protein patterning technique using photolithography and surface-initiated polymerization. This method allows for specific biomolecule immobilization, preserving protein functionality and reducing nonspecific adsorption.

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Polymer Chemistry

Background:

  • Protein immobilization is crucial for biosensors and diagnostics.
  • Existing methods often involve harsh conditions that can denature proteins.
  • Developing mild and specific immobilization strategies is essential for maintaining biomolecule functionality.

Purpose of the Study:

  • To develop a novel, mild protein patterning method.
  • To create surfaces with patterned poly(acrylic acid) (PAA) brushes for biomolecule attachment.
  • To demonstrate specific immobilization of proteins while minimizing nonspecific adsorption.

Main Methods:

  • Photolithography was used to pattern self-assembled monolayers of poly(ethylene glycol) (PEG) on silicon.
  • Surface-initiated atom transfer radical polymerization (ATRP) was employed to grow PAA brushes in patterned regions.

Related Experiment Videos

  • Biomolecules were covalently tethered to the PAA brushes under mild conditions, utilizing avidin-biotin interactions for specific immobilization.

    • Main Results:

    • Patterned PAA brushes were successfully synthesized at room temperature in an aqueous medium.
    • A variety of biomolecules were immobilized with reduced nonspecific adsorption due to surrounding PEG regions.
    • Avidin-biotin interaction enabled specific protein immobilization, preserving protein functionality.

    Conclusions:

    • The developed method offers a mild and effective approach for protein patterning and immobilization.
    • The combination of PAA brushes and PEG coatings provides a versatile platform for biosurface engineering.
    • This technique is suitable for applications requiring high protein activity and specificity.