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Related Experiment Videos

Human lymphocyte subpopulations: giant SRBC rosettes.

D T Yu

    Journal of Immunology (Baltimore, Md. : 1950)
    |June 1, 1976
    PubMed
    Summary

    Giant sheep red blood cell (SRBC) rosettes, defined as lymphocytes with ≥36 SRBCs, increase after mitogen stimulation. These giant rosettes serve as an early marker for activated lymphocytes, independent of cell size or DNA synthesis.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Human thymus-derived lymphocytes form rosettes with sheep red blood cells (SRBC) in vitro.
    • Giant SRBC rosettes (≥36 SRBCs per lymphocyte) are rare in normal peripheral blood.

    Purpose of the Study:

    • To investigate the nature and significance of giant SRBC rosettes.
    • To determine if giant SRBC rosettes are a marker for activated lymphocytes.

    Main Methods:

    • Peripheral blood lymphocytes from 10 normal subjects were assessed for giant SRBC rosette formation.
    • Lymphocytes were stimulated in vitro with four mitogens: sodium periodate, neuraminidase plus galactose oxidase, pokeweed mitogen, and concanavalin A.
    • Giant rosette formation was monitored over time, and compared with lymphoblast appearance and tritiated thymidine incorporation.

    Main Results:

    • Giant SRBC rosettes significantly increased 4-24 hours after mitogen stimulation, preceding lymphoblastogenesis and increased DNA synthesis.
    • The phenomenon was not due to mitogen adsorption, as receptors regenerated after trypsinization in vitro.
    • Giant rosette formation was independent of cell size increase or DNA synthesis.

    Conclusions:

    • Giant SRBC rosettes are a marker for activated lymphocytes.
    • The receptors involved in giant rosette formation are intrinsic to lymphocytes and not caused by adsorbed mitogens.
    • Giant SRBC rosette formation is an early indicator of lymphocyte activation.

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