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Related Concept Videos

Skeletal Muscle Anatomy00:55

Skeletal Muscle Anatomy

Skeletal muscle is the most abundant type of muscle in the body. Tendons are the connective tissue that attaches skeletal muscle to bones. Skeletal muscles pull on tendons, which in turn pull on bones to carry out voluntary movements.

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Related Experiment Video

Updated: Jul 11, 2026

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
08:38

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Published on: March 19, 2013

Tissue engineering of skeletal muscle.

Wentao Yan1, Sheela George, Upinder Fotadar

  • 1Department of Basic Sciences, New York University, New York, New York 10010, USA.

Tissue Engineering
|September 21, 2007
PubMed
Summary

Researchers developed a 3D skeletal muscle culture using satellite cells. This engineered muscle mimics native tissue structure and function, offering a potential solution for muscle reconstruction.

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Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

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Fabrication of Myogenic Engineered Tissue Constructs
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Fabrication of Myogenic Engineered Tissue Constructs

Published on: May 1, 2009

Related Experiment Videos

Last Updated: Jul 11, 2026

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
08:38

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation

Published on: March 19, 2013

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
09:30

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Published on: February 18, 2021

Fabrication of Myogenic Engineered Tissue Constructs
13:43

Fabrication of Myogenic Engineered Tissue Constructs

Published on: May 1, 2009

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Cell Biology

Background:

  • Skeletal muscle loss significantly impacts patient health, with no current replacement options.
  • Reconstructing functional muscle tissue requires in vitro models that replicate in vivo organization.

Purpose of the Study:

  • To develop a 3D skeletal muscle culture system using isolated satellite cells.
  • To create a prosthesis for reconstructing dysfunctional muscular tissue.

Main Methods:

  • Utilized neonatal satellite cells to construct multilayered skeletal muscle cultures.
  • Organized cells in a 3D pattern mimicking intact tissue.
  • Analyzed myotube formation, histology, gene expression, and physiological function.

Main Results:

  • Successfully constructed multilayered skeletal muscle cultures with 3D organization.
  • Myotubes were elongated, multinucleated, and MyoD positive.
  • Detected muscle-specific markers near adult levels via real-time RT-PCR.
  • Engineered muscle demonstrated tetanization and physiological force-length behavior.

Conclusions:

  • The developed 3D skeletal muscle culture system successfully mimics native tissue features.
  • This engineered muscle holds promise for future skeletal muscle tissue reconstruction and prosthetics.