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Updated: Jul 11, 2026
09:25
Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
Published on: June 2, 2021
Novel mating type-dependent transcripts at the mating type locus in Magnaporthe oryzae
Masaki Kanamori1, Hana Kato, Nobuko Yasuda
1Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, Japan.
Gene
|September 21, 2007
Summary
The mating type locus (MAT1) in Magnaporthe oryzae contains novel genes, MAT1-1-3 and MAT1-2-2, influencing mating. Sequence analysis reveals these genes share primary structures but differ in N-termini and upstream repeats.
Area of Science:
- Mycology
- Genetics
- Molecular Biology
Background:
- The mating type locus (MAT1) is crucial for sexual reproduction in ascomycetes.
- Magnaporthe oryzae, a significant fungal pathogen, possesses a MAT1 locus with conserved structural organization.
Purpose of the Study:
- To investigate the complete gene content and organization of the MAT1 locus in Magnaporthe oryzae.
- To identify and characterize novel open reading frames (ORFs) within the MAT1 locus.
Main Methods:
- Sequence analysis of the MAT1 locus.
- Expression analysis of identified ORFs.
- Comparative analysis of predicted protein structures.
Main Results:
- Identified known mating type genes (MAT1-1-1, MAT1-2-1) and novel ORFs (MAT1-1-2, MAT1-1-3, MAT1-2-2).
- MAT1-1-3 and MAT1-2-2 ORFs initiate within their respective idiomorphs or flanking regions, suggesting shared ancestry.
- Predicted proteins MAT1-1-3 and MAT1-2-2 exhibit similar primary structures but distinct N-termini.
- A polymorphic (CT)n repeat was found upstream of MAT1-1-3.
Conclusions:
- The MAT1 locus in Magnaporthe oryzae encodes additional regulatory or functional genes beyond the canonical mating type genes.
- The identified novel ORFs contribute to the complexity of mating-type determination and potentially sexual reproduction in this fungus.
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