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Updated: Jul 11, 2026
Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms
Published on: January 20, 2022
Validation of a PCR-oligochromatography test for detection of Trypanozoon parasites in a multicenter collaborative
F Claes1, S Deborggraeve, D Verloo
1Faculty of Bioscience Engineering, K U Leuven, Department of Biosystems, Kasteelpark Arenberg 30, 3001, Leuven, Belgium. filip.claes@biw.kuleuven.be
Abstract:
A PCR-oligochromatography test for diagnosis of human and animal trypanosomiasis was evaluated through a multicenter ring trial with six laboratories testing a set of 21 blinded samples, resulting in qualitative data (positive or negative). Results showed an intralaboratory repeatability (accordance) of 88.7% (credible interval [CI], 84.4 to 92.5%) and an interlaboratory repeatability (concordance) of 88.1% (CI, 84.3 to 92.3%).
