Related Experiment Video
Updated: Jul 11, 2026
Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
Published on: November 14, 2016
Molecular analysis of neurolysin expression in the rat and bovine ciliary body
Rubens Bertazolli-Filho1, Miguel Coca-Prados, Antonio Haddad
1Departamento de Biologia Celular e Molecular e Bioagentes Patogêcos, Faculdade de Medicina de Ribeirão Preto/USP, 14049-900 Ribeirão Preto, SP, Brazil. rbertazo@netscape.net
Purpose:
This paper deals with the capability of the ciliary epithelium to express neurolysin, involved in the inactivation of numerous neuropeptides.
Methods:
Total RNAs from ciliary body (CB) were processed for RT-PCR, and the amplification products were sequenced. The whole-protein extracts of CBs were analyzed using the Western blot. The CBs were processed for neurolysin immunolocalization.
Results:
The RT-PCR detected the presence of neurolysin mRNA in the ciliary body. The Western blot assays demonstrated immunochemical cross-reactivity with neurolysin. The immunoreactivity to neurolysin was observed in ciliary epithelium.
Conclusions:
These results indicate that the ciliary epithelium expresses neurolysin.
