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Updated: Jul 11, 2026
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Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes
Published on: August 12, 2017
Structure and function of embryonic rat retinal sheet transplants.
Qing Peng1, Biju B Thomas, Robert B Aramant
1Department of Ophthalmology, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, People's Republic of China.
Current Eye Research
|September 21, 2007
Summary
Retinal sheet transplants in degenerate rats restored light sensitivity and survived without immune rejection for 3 months. Histology confirmed well-developed photoreceptors and some integration with the host retina, suggesting functional restoration.
Area of Science:
- Ophthalmology
- Regenerative Medicine
- Neuroscience
Background:
- Inherited retinal dystrophies cause progressive vision loss.
- Retinal sheet transplantation is a potential therapeutic strategy.
- S334ter-line-3 rats serve as a model for inherited retinal degeneration.
Purpose of the Study:
- To evaluate the efficacy of retinal sheet transplants in S334ter-line-3 rats.
- To compare electrophysiological visual responses with morphological outcomes.
- To assess the survival and integration of transplanted retinal tissue.
Main Methods:
- Retinal sheets from embryonic day 19 (E19) rats were transplanted into the subretinal space of S334ter-line-3 rats.
- Electrophysiological recordings assessed visual responses in the superior colliculus.
- Light and electron microscopy evaluated transplant morphology and host-retinal integration.
Main Results:
- Transplanted rats showed visual responses to low light stimulation after 84-121 days.
- Histology revealed well-laminated transplants with photoreceptors and correct polarity.
- Electron microscopy indicated some integration between host and graft tissues.
Conclusions:
- Retinal sheet transplants survived without immune rejection and restored visual function for at least 3 months.
- The transplants contained well-developed photoreceptors and synapses.
- Evidence suggests integration and contribution of transplanted photoreceptors to restored light sensitivity.
