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Published on: December 14, 2011
Comparative proteomic analysis of human adenomyosis using two-dimensional gel electrophoresis and mass spectrometry
Haiyuan Liu1, Jinghe Lang, Xiaorong Wang
1Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing, China.
Objective:
To identify the differently expressed proteins in adenomyotic tissue compared with normal uterine muscle.
Design:
Retrospective, case-controlled study.
Setting:
University-based Department of Obstetrics and Gynecology and Proteomic Research Center.
Patient(S):
Ten patients who underwent transabdominal hysterectomy were included in this study. Five of them were histologically diagnosed with adenomyosis and the other five age-matched patients had cervical carcinoma in situ.
Intervention(S):
Postoperative adenomyotic tissue and normal uterine muscle samples were collected.
Main Outcome Measure(S):
The protein extracts from paired sample tissue were separated using two-dimensional gel electrophoresis. The differently expressed protein spots were detected by gel comparison using image analysis software and identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry.
Result(S):
Compared with protein profiling of normal uterine muscle, there were 12 protein spots dysregulated in adenomyotic tissue. Among them, 10 protein spots were successfully identified by mass spectrometry.
Conclusion(S):
Comparative proteomic analysis is a useful approach for the discovery of protein biomarkers for adenomyosis and understanding the pathophysiology of the disease.
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