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Updated: Jul 11, 2026
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Antibiotic susceptibility testing of Bifidobacterium thermophilum and Bifidobacterium pseudolongum strains: broth
Konrad J Domig1, Sigrid Mayrhofer, Ulrike Zitz
1BOKU-University of Natural Resources and Applied Life Sciences, Department of Food Science and Technology, Division of Food Microbiology and Hygiene, Gregor Mendel Str. 33, A-1180 Vienna, Austria. konrad.domig@boku.ac.at
Abstract:
There is urgent need for having available suitable methods and data regarding the susceptibility levels of antibiotic resistant and sensitive strains of bifidobacteria. Based on a defined standard operation procedure, agar disc diffusion and broth microdilution were compared in order to evaluate the antimicrobial susceptibility profiles of 82 B. pseudolongum and 80 B. thermophilum strains mainly originating from the meat production chain. The methods that were assessed showed interpretable agreement within this study. The disc diffusion zone diameters are highly reproducible making the method a useful alternative to broth microdilution for antimicrobial susceptibility screening of bifidobacteria.
