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Updated: Jul 11, 2026

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
Published on: June 5, 2020
[Dynamic analysis of HBV cccDNA in HepG2 cells infected with Ad-1.2 HBV]
Wei-fang Liang1, Zhi-hua Liu, Jie Yang
1Department of Infectious Diseases, Nanfang Hospital, Southern medical University, Guangzhou 510515, China.
Objective:
o study the replication of hepatitis B virus (HBV) in HepG2 cells infected with Ad-1.2 HBV.
Methods:
HepG2 cells were transfected with adenovirus containing 1.2 copies of HBV DNA. The expression of HBV antigens were detected in the culture medium by means of enzyme-linked immunosorbent assay (ELISA), and the covalently closed circular DNA (cccDNA) in the cells was extracted with plasmid extraction kit and detected by real-time PCR with selective primer after treatment with mung bean nuclease.
Results:
HBsAg, HBeAg and HBV cccDNA were all detected in HepG2 cells after tranfection with Ad-1.2 HBV. HBV cccDNA was detected 1 day after the infection, reaching the peak level 4 days after infection.
Conclusion:
Ad-1.2 HBV-infected cells can serve as the model for screening and evaluation of antiviral agents.
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