Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rap1-mediated steric hindrance protects telomeres from MRX sensing.

Nature structural & molecular biology·2026
Same author

SIRT3 deSUMOylation sustains hematopoietic stem cell activities under stressful conditions via the DHX58-IRF7 axis.

Haematologica·2026
Same author

The adaptive molecular landscape of reprogrammed telomeric sequences.

Nature communications·2026
Same author

Molecular evolution of animal aging.

The EMBO journal·2026
Same author

TRF2 Upregulates Protein Phosphatase 2A Subunit PPP2R2C to Attenuate DNA Damage Response at Telomeres and Pericentromeres Upon Replicative Stress.

Aging and disease·2026
Same author

Tel1 is recruited at chromosomal loop/axis contact sites to modulate meiotic DNA double-strand breaks interference.

PLoS genetics·2025
Same journal

Plucking cellular ribosomes with Ribo-Tweezer.

Nature reviews. Molecular cell biology·2026
Same journal

COPII meets autophagy at the ER membrane.

Nature reviews. Molecular cell biology·2026
Same journal

Diapause presses pause on life's developmental and ageing clock.

Nature reviews. Molecular cell biology·2026
Same journal

Histone acetylation at the dawn of gene regulation.

Nature reviews. Molecular cell biology·2026
Same journal

Regulation and function of specialized membrane protrusions in intercellular communication.

Nature reviews. Molecular cell biology·2026
Same journal

Ancient enzymes, new biotechnology applications.

Nature reviews. Molecular cell biology·2026
See all related articles

Related Experiment Video

Updated: Jun 25, 2026

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

How telomeres are replicated.

Eric Gilson1, Vincent Géli

  • 1Laboratoire de Biologie Moléculaire et Cellulaire, UMR5239, IFR 128, Centre National de la Recherche Scientifique, University Lyon 1, Faculty of Medicine Lyon-Sud, Hospices Civils de Lyon, Ecole Normale Supérieure de Lyon,France. eric.gilson@ens-lyon.fr

Nature Reviews. Molecular Cell Biology
|September 22, 2007
PubMed
Summary
This summary is machine-generated.

Replicating chromosome ends (telomeres) requires coordinated DNA synthesis. This process involves a synergy between replication machinery, protection systems, and DNA repair pathways for genome integrity.

More Related Videos

Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers
11:21

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

Published on: August 30, 2024

Related Experiment Videos

Last Updated: Jun 25, 2026

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers
11:21

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

Published on: August 30, 2024

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Linear chromosome ends, or telomeres, present unique replication challenges.
  • Maintaining telomere length is crucial for genome stability and cell viability.

Purpose of the Study:

  • To compare the mechanisms and timing of telomere replication initiation, control, and coordination.
  • To investigate leading and lagging strand synthesis at telomeres across different species.

Main Methods:

  • Comparative analysis of telomere replication processes.
  • Examination of DNA replication machinery, telomere protection, DNA-damage-response pathways, and chromosomal organization.

Main Results:

  • Identified specific mechanisms governing telomere replication in yeasts, ciliates, and mammals.
  • Highlighted the timing and coordination of leading and lagging strand synthesis at telomeres.

Conclusions:

  • Telomere replication is a complex process requiring a synergistic interplay of multiple cellular systems.
  • This synergy ensures genome integrity and facilitates cell division.