Three-dimensional refractive index reconstruction with quantitative phase tomography
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Updated: Jul 11, 2026
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
N M Dragomir1, X M Goh, A Roberts
1School of Physics, The University of Melbourne, Parkville 3010, Victoria, Australia. n.dragomir@physics.unimelb.edu.au
Abstract:
Optical tomography based on quantitative phase microscopy is used to determine nondestructively and with high spatial resolution the three-dimensional (3D) refractive index distributions within optical fiber devices. After obtaining a series of phase images of the fiber as it is rotated around its longitudinal axis at regularly-spaced angular positions, filtered backprojection is used to reconstruct a 3D map of the refractive index. The 3D refractive index distribution of the join region between two fusion spliced optical fibers is reconstructed with accuracy better than 10(-3).
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