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Updated: Jul 11, 2026
Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy
Published on: April 9, 2017
[Subcellular localization of APOBEC3G by confocal laser scanning microscope (CLSM)]
Yi-Shu Yang1, Lan Li, Ze-Lin Li
1College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100022, China. yishu-y@bjut.edu.cn
Abstract:
Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G) cDNA was amplified from total RNA prepared from nonpermissive H9 cells by RT-PCR. APOBEC3G cDNA is 1155nt long, encoding 384 amino acids. The APOBEC3G gene was then cloned into the eukaryotic expression vector pEGFP-C3. The generated pEGFP-3G construct was then transfected into CD4+ HeLa cell to determine the expression and the subcellular localization of GFP-APOBEC3G fusion protein. Under CLSM the localization of the expressed GFP-APOBEC3G in the cytoplasm of CD4+ HeLa cells was observed.
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