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Updated: Jul 11, 2026

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Published on: December 31, 2019
Beta- and gamma-di- and tripeptides as potential substrates for the oligopeptide transporter hPepT1
Ina Hubatsch1, Per I Arvidsson, Dieter Seebach
1Department of Pharmacy, Uppsala University, Box 580, SE-751 23 Uppsala, Sweden. ina.hubatsch@farmaci.uu.se
Abstract:
The hPepT1-mediated transport properties of a series of 11 synthesized beta- and gamma-peptides have been studied in Caco-2 cells. The results show that several of the compounds interact with the peptide transporter, but only two beta-dipeptides act as substrates and are transported across the cell monolayers. These two are less-efficient substrates than alpha-peptides. Larger derivatives than beta-dipeptides do not act as hPepT1 substrates, but instead, they appear to be substrates for P-glycoprotein efflux.
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