Search research articles

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Overexpression of <i>magA</i> in <i>Acidithiobacillus ferrooxidans</i> Increases Magnetosome Production and Pyrite Bioleaching.

ACS synthetic biology·2026

Same author

Protein Engineering of a Genetically Encodable Biosensor for Wastewater Detection of Profen NSAIDs.

Biotechnology and bioengineering·2026

Same author

Aqueous Depolymerization of Polyethylene at Ambient Temperature: <i>In Situ</i> Generation of Permanganate Using Ozone.

ACS sustainable chemistry & engineering·2026

Same author

Continuous Evolution of Calmodulin for High-Purity Separation of Rare Earth Elements.

Angewandte Chemie (International ed. in English)·2026

Same author

Rare Earth Element-Induced Condensation of the Block V of the Repeats-in-Toxin Domain from CyaA from <i><i>Bordetella pertussis</i></i> for Separations.

Langmuir : the ACS journal of surfaces and colloids·2025

Same author

Extremophilic microbes tolerate high concentrations of rare earth elements (REEs).

Biotechnology letters·2025

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 11, 2026

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

Bioactive proteinaceous hydrogels from designed bifunctional building blocks.

Ian R Wheeldon¹, Scott Calabrese Barton, Scott Banta

¹Department of Chemical Engineering, Columbia University in the City of New York, New York, NY 10027, USA.

Biomacromolecules

|September 25, 2007

Summary

This summary is machine-generated.

Researchers developed novel "smart" protein-based hydrogels using genetically engineered fluorescent proteins. These robust, active hydrogels offer tunable properties and broad bioengineering applications.

More Related Videos

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Published on: August 4, 2017

Related Experiment Videos

Last Updated: Jul 11, 2026

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Published on: August 4, 2017

Area of Science:

Biomaterials Science
Protein Engineering
Bioengineering

Background:

Stimulus-responsive protein-based hydrogels are crucial for bioengineering but often lack independent biological activity.
Existing hydrogels primarily focus on structural functionality, limiting their application scope.

Purpose of the Study:

To genetically engineer bifunctional protein building blocks for creating robust, active, and stimulus-responsive hydrogels.
To develop a versatile platform for constructing complex hydrogels with tunable fluorescence and mechanical properties.

Main Methods:

Genetic engineering of bifunctional protein building blocks incorporating fluorescent proteins.
Self-assembly of protein building blocks via native protein-protein interactions and alpha-helical aggregation.
Fluorescence Resonance Energy Transfer (FRET) experiments to analyze hydrogel structure and reactions.

Main Results:

Successful creation of robust and active hydrogels from engineered fluorescent protein building blocks.
Demonstrated independent tuning of fluorescence loading and hydrogel strength by mixing different fluorescent protein building blocks.
FRET analysis indicated a macro-homogeneous structure and the potential for engineering intragel and interprotein reactions.

Conclusions:

The novel design approach enables facile construction of complex, stimulus-responsive protein-based hydrogels.
These engineered hydrogels possess both structural integrity and biological activity, expanding their bioengineering potential.
The tunable nature of these hydrogels makes them suitable for a broad range of advanced applications.